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The carbonyl placement in the oxyanion hole likely positions the electrophilic center in the proper orientation for nucleophilic attack by Cys95 and explains why alternative warheads are not useful for this scaffold against UCHL3

Although the molecular mechanisms underpinning anhydrobiosis are not yet completely understood, it seems clear that desiccation tolerance depends on the synergic action of an array of many different molecules working together

It is recommended to start with a low dose of the peptide combination and gradually increase it over time in order to minimize the risk of side effects