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Double-blind, randomized trial comparing the efficacy and tolerability of fulvestrant versus anastrozole in postmenopausal women with advanced breast cancer progressing on prior endocrine therapy: results of a North American trial

In brief, enzyme conjugate (200 l) was added to 25 l of sample and standard (0, 0.2, 0.5, 1, 2, 6, and 16 ng/ml) well, mixed and incubated at room temperature (RT) for one hour

The pooled lentiviral supernatant was filtered through 0.45 m PVDF syringe filters (Whatman, 6746-2504), aliquoted and stored at 80 C