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To simulate extreme acidic conditions, PBS (pH=1) was used as the positive control group (PC)

The latter mechanism seems less probable because inhibition of absorption was not observed with the stronger chelator D-penicillamine

Thats because peptides can easily break down in the digestive tract and are often too molecularly large to permeate the intestinal wall.20 Oral alternatives may use technologies like protective capsules and permeability agents to bypass these obstacles, but injection remains the most common peptide delivery method.20 Collagen peptides are an exception