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Immunoblots Cells or tissues were lysed in buffer (150 mM NaCl, 10 mM Tris pH 7.6, 0.1% SDS and 5 mM EDTA) containing protease and phosphatase inhibitors (Thermo Fisher Scientific, 78445)

The occurrence of AE was not different between patients with active versus inactive preexisting AID, but fewer AE were seen in patients receiving therapy for their AID at the time of ICI initiation