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This involved perfusing the brains with phosphate-buffered saline followed by 4% paraformaldehyde, sectioning them into eight-micrometer coronal slices, and visually confirming the cannulas location within the left lateral ventricle [1]

Membranes were blocked overnight in PBS containing 0.1% Tween-20 and 5% non-fat dry milk

One commenter noted that the calculation included hospitals with fewer than three Safety of Care measures in the distribution and suggested that only hospitals with at least three measures be considered when calculating the quartiles to produce a more accurate assessment of performance, reduce volatility, and increase comparability in future public reporting

Scientific studies reporting on the possible anti-inflammatory effects of rooibos and/or its flavonoids are sparse