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The HPLC was set at a flow rate of 0.25 ml min 1 with 99% buffer A from 0 to 3 min, a linear gradient to 99% buffer A/1% buffer B (100% methanol) from 3 to 20 min, 80% buffer A/20% buffer B from 20 to 21 min, a linear gradient to 30% buffer A/70% buffer B from 21 to 28 min at 0.35 ml min 1 , 99% buffer A/1% buffer B from 28 to 31 min and a linear gradient to 99% buffer A from 31 to 37 min at 0.25 ml min 1

Whether they reflect dose-related issues, sourcing variability, or true pharmacological effects is unknown, precisely because the human data does not exist to answer that question

Finally, Kigelia oil is rich in flavonoids, steroidal saponins and phytosterols, essential for toning skin and muscles

This positioning makes it vulnerable to inflammation, fraying, and tears