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The supernatants were further clarified through a 0.45-m filter and applied onto GSTrap FF columns (GE Healthcare Life Sciences), which had been equilibrated with buffer B

doi: 10.1016/j.heliyon.2023.e19763 202 RodriguezJBystrynJC

Furthermore, a 2-year, double-blind, randomized, placebo-controlled therapeutic trial in 158 healthy siblings of hyperhomocysteinemic patients with premature atherothrombotic disease folic acid plus vitamin B 6 treatment was associated with a decreased occurrence of abnormal exercise electrocardiography tests, which is consistent with a decreased risk of atherosclerotic events (Vermeulen et al, 2000)

Loss to follow-up in trials of psychosocial intervention for autistic children tends to be low