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We discovered that SA--gal staining and quantification were significant lower in intervention groups than in placebo group, using an in-house quantification method (Image J) ( p = 0.001) (Figure 2B)

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To determine the optimal in vivo dose of 48c for our study, based on previously reported doses 33,34,35,36,37 , we treated the mice with 20 or 40 mg/kg 48c and found that although 20 mg/kg 48c efficiently inhibited Tm-induced Xbp1s expression, 40 mg/kg was required to increase Gclc expression level in the kidney (Supplementary Fig