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The identification and counting of CK18 + CD45 CTCs, as well as the examination of SOX2 levels in CTCs, were conducted through IF staining using the following primary antibodies: anti-CD45 (1:200, 60287-1-Ig, Proteintech), CoraLite Plus 488-conjugated anti-CK18 (1:200, CL488-66187, Proteintech), and CoraLite Plus 647-conjugated anti-SOX2 (1:200, CL647-66411, Proteintech)

This study was supported by grants to Szu-Tah Chen from the Chang Gung Memorial Hospital (CMRPG3H0411, CMRPG3H1261 and CMRPG3J1741) and a grant to Mei-Chich Hsu from the Ministry of Science and Technology of Taiwan (NSC101-2410-H-037-016-MY3)

For example, sulfasalazine, a clinically used ferroptosis inducer, is very effective against thyroid cancer cells, indicating its possible use in TAO (367, 368)

[] Controlled The randomized, double-blind, and placebo-controlled 12-week study evaluating the effectiveness of NAC in treating OCD showed a progressive and continuous reduction in the severity of disease symptoms in 19 patients (age: 2344) following treatment with NAC (titrated from 600 mg daily to 2.4 g daily at week 3) compared to the placebo ( n = 20)