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Point scanning excitation was performed at 488 nm using a pulsed white-light laser with emission collected over a bandwidth of 550650 nm onto a hybrid single molecule detector (HyD SMD) in single photon counting mode with a pinhole set to 1 A.U

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doi:10.1016/j.heliyon.2017.e00251

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Cellular components of the BBB can be distinguished by several antibodies ( Table 1 )

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