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For the 293T cell caspase-1 cleavage assays, 10 cm tissue culture plates of confluent 293T cells were transfected with 10 g murine Il1b and point mutant variants, as described above, and after 24 h cells harvested and plated onto 24-well tissue culture plates

Western blots are useful because specific bands can be visualized (stained) on the nitrocellulose membrane using a detection system linked to an antibody that recognizes a specific target protein

In metabolic disease contexts, studies have found that supraphysiological doses of coenzyme Q 10 (CoQ 10 ) can specifically upregulate SQOR expression and simultaneously inhibit key enzymes (CBS and CSE) in the transsulfuration pathway, thereby reshaping the serinefolate cycle and nucleotide metabolism (Gonzlez-Garca et al., 2020)

10.1152/ajplung.00098.2012 4 Alajbegovic-HalimicJ.ZvizdicD.Alimanovic-HalilovicE.DodikI.DuvnjakS