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Additionally, carvacrol and cilostazol co-administration was found to ameliorate ethanol-induced liver fibrosis through the SIRT1/Nrf2/HO-1 pathway, exerting antioxidant, anti-inflammatory, and anti-apoptotic effects ( Lastly, biliary senescence and hepatic fibrosis are central features of cholangiopathies, such as primary sclerosing cholangitis (PSC)

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Viability was assessed by Zombie UV Fixable Viability Dye (BioLegend) and cytokine production was measured by intracellular cytokine staining after 3 h stimulation with PMA (50 ng ml 1 ) (BioGems) and ionomycin (1 g ml 1 ) (BioGems) in the presence of brefeldin A (1 g ml 1 ) (GolgiPlug, BD Biosciences)

Recent EMG research challenges this belief