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Expression of N-terminal His-tagged human GCLM was induced with 1 mM isopropyl--D-thiogalactoside (IPTG, Wako Pure Chemical Industries, Ltd., Osaka, Japan) at 30C for 5 h in Escherichia coli

In rabies virus G protein, two modalities of neutralization were described: (1) targeting an epitope on a single domain that is fully accessible only in prefusion is enough to lock the protein in prefusion conformation, hence inhibiting the fusion process 44 and (2) recognition of an epitope that spans more than three domains, including one on an adjacent protomer, prevents the conformational changes required for fusion 45

This is a distinct entity from acidosis that results from paracetamol toxicity, which is due to liver derangement and lactic acidosis