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The body interprets rapid fat loss as a significant stressor, releasing inflammatory mediators as part of the adaptive response

Immunofluorescence staining of brain tissue examined the expression of tumor necrotic factor-alpha (TNF-, pro-inflammatory cytokine), glial fibrillary acidic protein (GFAP, a marker of activated astrocytes), sequestosome 1 (p62, marker of autophagic activity), voltage-dependent anion channel 1 (VDAC1, marker of mitochondrial stress), and NADPH Oxidase 4 (NOX4, marker of mitochondrial stress)

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When genes related to the initiation of apoptosis are deleted, developing tissue cannot form properly, leading to neurodevelopmental issues such as spina bifida, improper neural tube closure, deficient removal of interdigital webs, and other tissue malformations (Kuida et al., 1996