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Chromatin immunoprecipitation assays ChIP experiments were conducted using ChIP-IT High Sensitivity Kit (ab185913, Abcam) with adaptations

17, 66 Mice deleted of claudin-1 protein expression die premature owing to dehydration, suggesting a key function for this protein in regulating dermal barrier function

Results Method Development For unbiased analysis of binding to cysteines in recombinant GSTs, each GST was probed for covalent GSH-cysteine adducts, which could have occurred during GST purification over GSH affinity chromatography

The chronic inflammatory environment degrades existing collagen faster than the body can replace it, and the disorganized keratinocyte proliferation further disrupts normal tissue structure