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In contrast to PTT, PDT utilizes the hypoxia-cleavable moieties to convert light energy into ROS and in some cases, this leads to increase in the hypoxia conditions of the cancers

Likewise, a synthetic gene for mScarlet3 42 carrying the N-terminal Azo-tag directly after the start Met residue (for cytoplasmic expression) was subcloned via the Nde I and Afe I restriction sites