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The term affinity chromatography introduced in 1968 by Pedro Cuatecasas, Chris In an article, Anfinsen and Meir Wilchek briefly described the enzyme method purification via immobilized substrates and inhibitors

SAMe is one of the main donors of methyl groups to methyl transferase enzymes

Two key pathogenetic changes in the synovium in RA are an increase and activation of synoviocytes ( A variety of risk factors are involved in the development of RA, including genetic and environmental factors

The sonicated lysate was centrifuged and diluted using 10 volumes of cold ChIPdilution buffer (0.01% SDS, 1.1% Triton X100, 1.2 mM EDTA, 16.7 mM TrisHCl, pH 8, 167 mM NaCl)