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I tried increasing the frequency of SI from 2 weekly to 1 weekly and the flushing got much worse - so I think this is the likely culprit

This can be especially beneficial if youre experiencing stress-related cognitive issues

qPCR was performed as 10 L reaction mix with a final concentration of 1 SYBR Green (Roche), 0.5 L of each primer, and the cDNA that was synthesized in the earlier step