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Description

10.1016/j.bmc.2010.12.026 49 GersonS

doi: 10.1007/s00262-018-2120-5

The rescue plasmid containing full mamA gene sequence with two point mutations at nucleotides 96 (C G) and 99 (C T) of the coding sequence was done in the pSS047 backbone, which integrates at the Giles attB site and is derived from pGH1000A [12] using the native mamA promoter and 5 UTR (242 nt upstream of the coding sequence: 42 nt known UTR and 200 nt predicted promoter)