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Description

The rhodanese/Cdc25 phosphatase superfamily

Guijarro LG, Cano-Martnez D, Toledo-Lobo MV, Ruiz-Llorente L, Chaparro M, Guerra I

Tissues were weighed then homogenized using Qiagens TissueLyzer in 800 l ice-cold 50:50 methanol:water solution per 50 mg tissue, with BIS-TRIS (Sigma-Aldrich) at 125 ng/ml was used as an internal control, in 2 ml microtubes containing one 3 mm Tungsten carbide bead (Qiagen), for 10 min at a frequency of 25 Hertz