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Studies showed that Bmal1 and Sirt1 mRNA expressions were highly correlated, therefore suggesting mutual positive regulation [32]

Cells were then washed twice with Hanks Balanced Salt Solution (HBSS, H8264) and incubated with 200 L GDC (12 concentrations, 05 mM) in DMEM D1145 or 1% TritonX (X-100)