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doi: 10.1172/JCI113366

The antibodies used were as follows: anti-SIRT1 (AF1267, Beyotime, shanghai, China), anti-BDNF (AF1423, Beyotime, shanghai, China), anti-TrkB (131291-AP, proteintech, Sanying, Wuhan, China), anti-MAPK (AF5884, Beyotime, shanghai, China), anti-p53 (AF7671, Beyotime, shanghai, China), anti-p21 (AG8586, Beyotime, shanghai, China), anti-FOXO1A (AF1600, Beyotime, shanghai, China), anti-CDK2 (AF1063, Beyotime, shanghai, China), anti-caspase-3 (AF0081, Beyotime, shanghai, China), anti-BCL2-associated X (BAX, AG1208, Beyotime, shanghai, China), anti-BCL2 apoptosis regulator (BCL2,AF6285, Beyotime, shanghai, China), anti-cyclin D1 (AF0126, Beyotime, shanghai, China), anti-C-MYC (AF6513, Beyotime, shanghai, China), anti-PI3K (AF7749, Beyotime, shanghai, China), anti-protein kinase B (AKT, AF1777, Beyotime, shanghai, China), and anti-GAPDH (1:1,000 dilution, AF0006, Beyotime, shanghai, China), goat anti-rabbit and goat anti-mouse HRP-coupled secondary antibodies (1:5,000 dilution, BOSTER)

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By April 1946, peak demand for water in the city had reached 59 million gallons a day