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The abnormal cholesterol profile in patients with preclinical RA and early RA is typical of metabolic syndrome: normal or mildly elevated total cholesterol, LDL cholesterol and triglycerides, associated with decreased HDL cholesterol levels (122, 123)

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While stress + UFe treatment resulted in moderate enrichment of key discriminative metabolites, CK-stress samples consistently exhibited the lowest normalized values, underscoring the suppressive effect of saline-alkali stress in the absence of nanoparticles intervention