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Accordingly, we found that artificial elevation of glutathione levels may induce SA accumulation in tobacco leaves, either following a single infiltration of exogenous GSH or OTC or continuous exposure to OTC ( Figure 1B , Figures S1 and S2 ) Furthermore, although GSH and OTC treatments of SA-deficient NahG tobacco did not increase free SA levels, bound SA levels were unexpectedly (1.5 to 2-fold) higher in treated NahG plants

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Clinical trials and observational studies have demonstrated that nutritional interventions focusing on this pathwayparticularly using methylcobalamin and folinic acidmay lead to measurable improvements in behaviour, communication, and cognitive function for some children with autism, especially those with evidence of methylation pathway dysfunction