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The supernatant was filtered to remove phospholipids, dried, dissolved in 50% isopropanol, and used for the assay

Journal of Chromatography B: Biomedical Sciences and Applications, 496(2), 175-184

Twenty-six adults with major depressive disorder participated in a double-blind crossover study, receiving oral placebo or 50 mg naltrexone before an intravenous infusion of 0.5 mg per kg ketamine