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Conversion of acetaminophen to the bioactive N-acylphenolamine AM404 via fatty acid amide hydrolase-dependent arachidonic acid conjugation in the nervous system

After centrifugation, the supernatants were incubated with 20 l of anti-myc antibody bound to agarose or anti-HA antibody bound to agarose beads (Sigma) for 2 h at 4 C with rotation