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Western blotting The encephalons of 4-dpf larvae were dissected and lysed in RIPA lysis buffer supplemented with protease inhibitor cocktail (Roche, #04693116001) for Western blot analysis

Further examination with Rev-erb knockout mice, indicated increased basal nuclear factor B (NFB) and enhanced hippocampal neuroinflammatory reactivity after inflammatory challenge (Griffin et al., 2019) providing further evidence of the role and importance of circadian clock regulation on immune function

minimise extended exposure above 25C Compound-specific notes Copper-bound preparation typically presents as a blue lyophilized solid

Swirl gently, never shake