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glutamicum ( CgMCS gene) or Escherichia coli ( EcMCS gene) in NG13, introduced by pCMCS or pEMCS plasmids, respectively, provided similar glutamate production levels to that with the CgCS strain (Supplementary Fig

The concentrates were then resuspended with 4 l of 0.5% ProteaseMAX Surfactant (Promega), 4 l of 1 M ammonium bicarbonate, 1 l of 100 mM TCEP, and 10 l of distilled water, and then subjected to incubation in a shaker (2400 rpm) at 37 C for 15 min