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In support with this, several studies highlighted that cells experiencing hypoxia or mitochondrial dysfunction have decreased glutamine oxidation and rely on carbon sources, other than glucose and glutamine, to supply metabolic intermediates under hypoxic conditions 46,47,48

Alternatively, species may encode PRXs that are sensitive to hyperoxidation but that are not reactivated, given that reactivation may not always confer increased fitness

Bait proteins used for pull-down assays can be either ready-to-use purified proteins by linking an affinity tag or producing recombinant fusion-tagged proteins