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To this point, it should be mentioned, however, that there is also some caution about the use of peptidergic agents, and adaptation processes, particularly on a long-term basis [156]

We speculate that the peroxynitrite production by misfolded metallated SOD1 may benefit, similarly to the HO formation, by the ability of SOD1 enzyme to accept ubiquitous biological thiols as an alternative substrate for cooper activation

The full course of treatment varies but typically runs six months to one year before evaluating maintenance strategies

Buffers containing strong chelators (EDTA, EGTA) or reducing agents (DTT, -mercaptoethanol, TCEP) should be avoided at the reconstitution step, since chelators can strip copper from the GHK-Cu component