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ROS initiates increase of P2X7 expression on the mitochondrial membrane and activity of Complex I, hence increasing mitochondrial polarization, which leads to an elevation of a cascade of unfavorable processes: depolarization of the mitochondrial membrane, calcium overload, and release of cytochrome c , which ultimately leads to cell death by apoptosis way

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The HG + si-Nrf2 group (si-Nrf2 group) was cultured in medium containing 25 mmol/l glucose for 48 h, with the addition of 5 l of si-Nrf2 at a concentration of 20 M for 6 h, followed by culture in medium containing 5.5 mmol/l glucose for 48 h