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Description

Briefly, for Co-IP assays, 100 l of plasmid DNA (2 g l 1 ) was mixed with 1 ml of protoplasts (2 105 cells per ml) for PEG-mediated transfection

TB-500 Loading: 1,250 mcg twice weekly for 4 weeks (total ~2.5 mg/week)

The use of diet in the treatment of epilepsy

The cells were incubated at room temperature for 30 minutes in the dark, and the cell cycle was analyzed by flow cytometry (Win Bryte HS cytometer [Bio-Rad], using software Win Bryte, Bio-Rad Laboratories Inc., Hercules, CA, USA)