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If you changed five variables at once, you need a clean timeline to know what might have mattered

In the Boyden chamber assay, TB4 stimulated keratinocyte migration 23-fold over medium alone after just 45 hours, with as little as 10 pg of TB4 sufficient to produce a potent stimulatory response

10.1016/j.molimm.2018.10.016 122 HuangJ.-Y.PengS.-F.ChuehF.-S.ChenP.-Y.HuangY.-P.HuangW.-W.et al (2021)

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