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This demonstrates utility for bone tissue engineering applications, potentially addressing osteoporosis and bone healing challenges

[64] Of course, artifacts of cell culturee.g., removal of neurons from the influence of gliacould create spurious results as well

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Primary antibodies were diluted in TBST skimmed milk 5% buffer and the incubation was performed overnight at 4C using an orbital shaker followed by 2 h at room temperature with the secondary horseradish peroxidase (HRP)-linked antibody (Goat Anti-Mouse or Anti-Rabbit IgG Peroxidase Conjugated by Thermo Scientific)