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After incubation, the stain was removed and monolayers were washed, at least 3 times, with PBS (until the PBS was clear)

Compliance with Ethics Guidelines This article is based on previously conducted studies and does not contain any new studies with human participants or animals performed by the authors

It functions by blocking an enzyme known as nicotinamide N-methyltransferase (NNMT), which is important for how the body stores fat and uses energy

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