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The clarified supernatant was filtered through a 0.4 M filter to remove the insoluble fraction and supplemented with 30 mM Imidazole pH 7.0 immediately before loading at a flow rate of 2 mL/min onto a 5 mL Ni-NTA immobilized metal affinity chromatography (IMAC) column (Bio-Rad) equilibrated in Buffer A (50 mM Tris-Cl, 300 mM NaCl, 0.01% LMNG/0.001% CHS, pH 7.0)

Liposomal Glutathione: The Mighty Micronaut The second member of our dynamic duo, Liposomal Glutathione, takes a different approach to tackle the bioavailability conundrum

doi:10.1002/14651858.CD003101.pub3

The human equivalent dose for the cyano- and methyl-cobalamin drinking water used in these studies is ~5000 g/day (CNCbl10 & MeCbl10) and ~25,000 g/day (CNCbl40 & MeCbl40), which was calculated using the standard human equivalent dose parameters [18]