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The antigens were first prepared in buffered saline containing 5% bovine serum albumin for 20 min in a microwave with sodium citrate buffer (pH = 6)

Our team has dedicated more than 1,000 cumulative hours over the years researching various peptides, two of which are TB4 and one of its synthetic analogs, TB-500

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In the non-parametric Mann Whitney U test, eight biomarkers showed a significant difference, in the DCM-induced animals displaying a significant increase in: TG (SMD 16.60, p = 0.0007), BG (SMD 7.54, p = 0.00001), TC (SMD 7.38, p = 0.009), EF% (SMD -4.13, p = 0.00001), NF-KB (SMD 3.13, p = 0.0001), HMGB1 (SMD 3.00, p = 0.0001), FS% (SMD -2.91, p = 0.00001) and HW/BW ratio (SMD 2.31, p = 0.007)