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Description

The colored end-product of the assay demonstrated remarkable stability at room temperature, with the absorbance at 412 nm remaining stable for over three hours

For GSSG competition assay (Extended Data Figure 4l), the 2x reaction buffer consisted of 10LM [ 3 H]GSSG, 5mM GSSG, 150mM KCl, and 50mM HEPES (pH 7.4)

The main action of SOD is to reduce the damage caused by superoxide, the most common free radical, by converting superoxide into water and hydrogen peroxide