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As a result, a connection between secondary bile acids with inflammatory bowel disease, nonalcoholic fatty liver disease, and cancers of the liver, esophagus, and colorectum has been observed, although the potential role of bile acids in the establishment and progression of these diseases is still being investigated (43)

Dichloromethane (DCM) (Merck, Darmstadt, Germany) used for gas chromatography analysis was MS-SupraSolv grade

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