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Regulation of the pentose phosphate cycle

Cross-linking was performed as following: anti-IgG and anti-FLAG were incubated with pre-washed Dynabeads Protein G (ratio 50 g antibody with 500 l ProteinG) for 2 hrs at R.T., after washes, beads were resuspended in 20 mM DMP (dimethyl pimelimidate) (Thermo Fisher Scientific, #21666) in Borate Buffer (40 mM Boric acid, 40 mM Sodium tetraborate decahydrate) at R.T

Interestingly, while some of them were selective PAMs of 5-HT2CR, others were described as dual 5-HT2AR/5-HT2CR PAMs