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Then, 10 L of the CCK-8 solution was added to each well after incubation at 37 C for a fixed time and the samples were incubated further for 16 h

33 CirioloM

This type of tolerance is generally short-lasting, since (functionally) deleted lymphocytes are gradually replenished by newly arising clones in the bone marrow and thymus and, in experimental animal models, cannot be transferred to naive recipients, since these still contain a fully functional repertoire, compensating for any missing clones