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Int J Environ Sci Technology

Site in a sheltered location, as the wind may catch the heavy heads

Nayan Patel PharmD

The membrane was washed once with Tris-buffered saline with 0.1% Tween 20 (TBST) and incubated with a streptavidinalkaline phosphatase conjugate (KPL, medac, 1:2,000 in 1% BSA (Fraction V, Roche) in TBST) for 2 h at room temperature