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Following alkylation by add IAM (Iodoacetamide) for 40 min at room temperature under dark conditions

Opioid antagonists (naltrexone or nalmefene) and medications that modulate gamma-aminobutyric acid receptors (baclofen or acamprosate) appear to have a short-term benefit by reducing the craving and withdrawal symptoms

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Thermo Fischer Scientific), 30% glucose, 7.5% Na bicarbonate (Thermo Fischer Scientific), 100 mM l-glutamine (Merck KGaA), 2 mg/ml heparin (2 mg/ml), bovine serum albumin (BSA