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The cultivation was performed in 500 mL baffled shake flasks with 50 mL LB medium supplemented with antibiotics as stated above on a shaking incubator (37C, shaking frequency: 180 min 1 , eccentricity: 25 mm) with a starting OD 600 of 0.1

It has been observed that PD-1 blockade during primary infection increases viral loads, generates reactivations of other pathogens, increases tumor burden, and increases elevated production of immunosuppressive cytokines (301)

This age-related decline correlates with many hallmarks of aging, including reduced collagen production, slower wound healing, increased inflammation, and diminished tissue repair capacity