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Now that you understand the detoxification process, let's look at how PCOS, a hormonal imbalance, can disrupt this system

Acknowledgements We thank Astrid Joachimi, Anna Heiler, Yuanhao Li, Dennis Klge, Vera Hedwig, Malin Bein, Christoph Globisch, and the members of the Proteomics and NMR facilities of University of Konstanz for technical assistance and helpful discussions

Yengi L, Inskip A, Gilford J, Alldersea J, Bailey L, Smith A et al

Western blot analyses using the iBLOT System (Invitrogen) were performed on nitrocellulose membranes after blocking in 1 Rotiblock (Carl Roth) in TBS-T using the fluorescence-labelled anti-BNIP3 antibody 1C8 (200 ng ml 1 ) and anti-BAX antibody (2D2, 1:1000, Cell Signaling Technologies) overnight