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Int J Biol Macromol 98:664675

20,350 This phenomenon is evidenced by the significantly higher concentration of secondary BAs in the intestines and fecal samples of SPF mice compared to GF mice, providing strong evidence for their microbial origin

Histidine and lysine produced significantly more pigment than arginine under identical conditions, and a Box-protective lysine (Boc-Lys), which preserves the -NH 2 group, yielded deeper pigmentation than unprotected lysine, implicating the -amino group of lysine as a key reactive site