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Science 300 , 342344 (2003)

Leaves were cut with scissors and the slices incubated overnight in the dark at 23 C in F-Pin solution (10 mM MES pH 5.8, 0.32% Gamborgs B5 basal salt powder with bioWORLD vitamins, 0.4 M sucrose) containing 0.5% (w/v) cellulase Onozuka R10 and macerozyme R10 (SERVA Electrophoresis GmbH, Heidelberg, Germany)

In reality, the human body tightly regulates blood pH between 7.35 and 7.45, and dietary bicarbonate has negligible influence on this system

This aligns with the position of major medical organizations, including the American Cancer Society and National Cancer Institute, which do not recognize coffee enemas as having any proven therapeutic value