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Detection of mitochondrial ROS, apoptosis, and viability For mitochondrial ROS measurements, cells were stained with MitoSOX Red (2.5 M, 30 minutes, dark), washed with phosphate-buffered saline (PBS), detached using trypsin-EDTA, and washed twice in Hanks Balanced Salt Solution (HBSS) by centrifugation (5 min, 1200 rpm, room temperature)

To be better used as antiangiogenic agent, ISA has to be modified to lower the toxicity in animals shown by Zhu et al (Zhu Y

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