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The untreated and MSO-treated parasite pellets isolated by saponin treatment were pooled separately during solubilization with 6 M urea in 25 mM ammonium bicarbonate buffer

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Dev Comp Immunol 39:3962 Acknowledgements The authors would like to acknowledge the support of the Biotechnology Lab., Faculty of Aquatic and Fisheries Sciences, Kafrelsheikh University, Egypt, where the laboratory investigations were carried out

The 1870 census notes that Pearsall lived with his wifes wealthy parents in New York City and was a fruit dealer