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Generation of hMSC cells in the Ryan lab was supported by 19/FFP/6446Research Ireland Frontiers for the future award

The filter was washed two times with 100 L of UA, centrifuged at 14,000 g for 15 min, and incubated with 100 L of IAA solution (0.05 M iodoacetamide in UA) for 20 min in the dark